PFG-NMR diffusometry : A tool for investigating the structure and dynamics of noncommercial purified pig gastric mucin in a wide range of concentrations

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PFG-NMR diffusometry : A tool for investigating the structure and dynamics of noncommercial purified pig gastric mucin in a wide range of concentrations

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dc.contributor.author Lafitte, Geraldine
dc.contributor.author Söderman, Olle
dc.contributor.author Turesson, Krister
dc.contributor.author Davies, Julia
dc.date.accessioned 2010-12-17T10:45:09Z
dc.date.available 2010-12-17T10:45:09Z
dc.date.issued 2007
dc.identifier.citation 165-175 en
dc.identifier.issn 0006-3525 en
dc.identifier.uri http://hdl.handle.net/2043/11108
dc.description.abstract For the first time, Pulsed Field Gradient-Nuclear Magnetic Resonance, a powerful noninvasive tool for studying the dynamics and structure of complex gels, has been used to measure diffusion of probe molecules in aqueous solutions/gels of noncommercial purified pig gastric mucin (PGM), in a concentration range up to 5 wt %. Complementary data were obtained from rheology measurements. The combination of techniques revealed a strong pH dependency of the structure of the PGM samples while changes in concentration, ionic strength, and temperature appeared to induce less pronounced alterations. Viscosity was found to vary in a nonmonotonous way with pH, with the more viscous solutions found at intermediate pH. We propose that this finding is due to a reduced charge density at lower pH, which is expected to continuously increase the relative importance of hydrophobic associations. The results suggest a loose network of expanded fully charged PGM molecules with considerable mobility at neutral pH (pH 7.4). At intermediate pH (pH 4), a three-dimensional expanded network is favored. At pH 1, the charge density is low and microphase separation occurs since hydrophobic associations prevail. This leads to the formation of clusters concentrated in PGM molecules separated by regions depleted in PGM. The results obtained increase our knowledge about the gastric mucosal layer, which in vivo contains mucin in the same concentration range as that of the samples investigated here. en
dc.language.iso eng en
dc.publisher Wiley en
dc.subject PFG-NMR diffusometry en
dc.subject rheology en
dc.subject mucin gel en
dc.subject structure and dynamics en
dc.subject.classification Sciences en
dc.title PFG-NMR diffusometry : A tool for investigating the structure and dynamics of noncommercial purified pig gastric mucin in a wide range of concentrations en
dc.type Article, peer reviewed scientific en
dc.contributor.department Malmö University. Faculty of Odontology
dc.identifier.doi 10.1002/bip.20717 en
dc.subject.srsc Research Subject Categories::MEDICINE::Chemistry::Biochemistry en
dc.relation.ispartofpublication Bioploymers;2 en
dc.relation.ispartofpublicationvolume 86 en
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