The effects of antimicrobials on endodontic biofilm bacteria

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The effects of antimicrobials on endodontic biofilm bacteria

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dc.contributor.author Chávez de Paz, Luis Eduardo sv
dc.contributor.author Bergenholtz, Gunnar sv
dc.contributor.author Svensäter, Gunnel en_US
dc.date.accessioned 2011-01-13T14:00:45Z
dc.date.available 2011-01-13T14:00:45Z
dc.date.issued 2010
dc.identifier.issn 1878-3554 en
dc.identifier.uri http://hdl.handle.net/2043/11489
dc.description.abstract Introduction In the present study, confocal microscopy, a miniflow cell system, and image analysis were combined to test in situ the effect of antimicrobials and alkali on biofilms of Enterococcus faecalis, Lactobacillus paracasei, Streptococcus anginosus, and Streptococcus gordonii isolated from root canals with persistent infections. Methods Biofilms formed for 24 hours were exposed for 5 minutes to alkali (pH = 12), chlorhexidine digluconate (2.5%), EDTA (50 mmol/L), and sodium hypochlorite (1%). The biofilms were then characterized by using fluorescent markers targeting cell membrane integrity (LIVE/DEAD) and metabolic activity (5-cyano-2,3-ditolyl tetrazolium chloride and fluorescein diacetate). In addition, the biofilm architecture and the extent to which coating of the substrate surface with collagen influenced the resistance pattern to the chemicals were also analyzed. Results NaOCl (1%) affected the membrane integrity of all organisms and removed most biofilm cells. Exposure to EDTA (50 mmol/L) affected the membrane integrity in all organisms but failed to remove more than a few cells in biofilms of E. faecalis, L. paracasei, and S. anginosus. Chlorhexidine (2.5%) had a mild effect on the membrane integrity of E. faecalis and removed only 50% of its biofilm cells The effects were substratum-dependent, and most organisms displayed increased resistance to the antimicrobials on collagen-coated surfaces. Conclusions The biofilm system developed here was sensitive and differences in cell membrane integrity and removal of biofilm cells after exposure to antimicrobials commonly used in endodontics was discernible. en
dc.format.extent 8
dc.language.iso eng en
dc.publisher Elsevier en
dc.subject image analysis en
dc.subject substrate conditioning en
dc.subject Alkaline resistance en
dc.subject antimicrobial resistance en
dc.subject biofilm model en
dc.subject biofilm system en
dc.subject biofilms en
dc.subject environmental changes en
dc.subject microbial adaptation en
dc.subject persistent infections en
dc.subject.classification Medicine en
dc.title The effects of antimicrobials on endodontic biofilm bacteria en
dc.type Article, peer reviewed scientific en
dc.contributor.department Malmö University. Faculty of Odontology
dc.identifier.doi 10.1016/j.joen.2009.09.017 en
dc.subject.srsc Research Subject Categories::ODONTOLOGY::Endodontology en
dc.subject.srsc Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases::Microbiology en
dc.relation.ispartofpublication Journal of Endodontics;1 en
dc.relation.ispartofpublicationvolume 36 en
dc.format.ePage 77
dc.format.sPage 70
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