Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts

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Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts

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dc.contributor.author Schwartz Filho, Humberto Osvaldo en_US
dc.contributor.author Morandini, Ana Carolina Faria en_US
dc.contributor.author Ramos-Junior, Erivan Schnaider en_US
dc.contributor.author Jimbo, Ryo en_US
dc.contributor.author Santos, Carlos Ferreira en_US
dc.contributor.author Marcantonio, Elcio Jr en_US
dc.contributor.author Wennerberg, Ann en_US
dc.contributor.author Marcantonio, Rosemary Adriana Chiérici en_US
dc.date.accessioned 2012-12-21T08:26:46Z
dc.date.available 2012-12-21T08:26:46Z
dc.date.issued 2012 en_US
dc.identifier.issn 1549-3296 en_US
dc.identifier.uri http://hdl.handle.net/2043/14692
dc.description.abstract Implant topography is an important factor that influences many cell types. To understand the role of topography in the inflammatory events, we evaluated the response of human gingival fibroblasts (HGFs) by the release pattern of cytokines. HGFs were cultured on Ti discs for 24 and 48 h. Four different surface treatments were used: machining method (turned), blasting followed by an acid-etching method (BAE), oxidative nanopatterning (ON) method, and an association of blasting followed by an acid-etching plus oxidative nanopatterning (BAE+ON) method. Extracellular levels of IL-6, IL-8, transforming growth factor beta (TGF-β), IL-4, and IL-10 were measured by enzyme-linked immunosorbant assay. Increased levels of IL-6 and IL-8 were observed in all surfaces after 24 h which decreased after 48 h. BAE, ON, and BAE+ON surfaces showed a reduction in IL-6 levels compared with the turned after 48 h (p < 0.05). On one hand, IL-8 production was lower in BAE+ON in comparison to the turned surface (p < 0.05). On the other hand, IL-4 showed increased levels with 48 h, which were significantly different between turned, BAE, and ON surfaces, but not with BAE+ON. Additionally, TGF-β and IL-10 production were not detected. This study indicates that nanotopography might be important in the modulation of the inflammatory response in cultured HGFs. en_US
dc.format.extent 8
dc.language.iso eng en_US
dc.publisher Wiley
dc.subject Wiley en_US
dc.subject.classification Medicine en_US
dc.title Titanium surfaces with nanotopography modulate cytokine production in cultured human gingival fibroblasts en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Odontology
dc.identifier.doi 10.1002/jbm.a.34200 en_US
dc.subject.srsc Research Subject Categories::ODONTOLOGY en_US
dc.relation.ispartofpublication Journal of Biomedical Materials Research A;10
dc.relation.ispartofpublicationvolume 100 en_US
dc.format.ePage 2636
dc.format.sPage 2629
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