The effect of laminin-1-doped nanoroughened implant surfaces : gene expression and morphological evaluation

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The effect of laminin-1-doped nanoroughened implant surfaces : gene expression and morphological evaluation

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dc.contributor.author Schwartz Filho, Humberto Osvaldo sv
dc.contributor.author Bougas, Kostas sv
dc.contributor.author Coelho, Paulo G sv
dc.contributor.author Xue, Ying sv
dc.contributor.author Hayashi, Mariko sv
dc.contributor.author Faeda, Rafael Silveira sv
dc.contributor.author Marcantonio, Rosemary Adriana Chiérici sv
dc.contributor.author Ono, Daisuke sv
dc.contributor.author Kobayashi, Fumio sv
dc.contributor.author Kamal, Mustafa sv
dc.contributor.author Wennerberg, Ann en_US
dc.contributor.author Jimbo, Ryo en_US
dc.date.accessioned 2012-12-21T11:09:21Z
dc.date.available 2012-12-21T11:09:21Z
dc.date.issued 2012 en_US
dc.identifier.issn 1687-8787 en_US
dc.identifier.uri http://hdl.handle.net/2043/14706
dc.description.abstract Aim. This study aimed to observe the morphological and molecular effect of laminin-1 doping to nanostructured implant surfaces in a rabbit model. Materials and Methods. Nanostructured implants were coated with laminin-1 (test; dilution, 100 μg/mL) and inserted into the rabbit tibiae. Noncoated implants were used as controls. After 2 weeks of healing, the implants were removed and subjected to morphological analysis using scanning electron microscopy (SEM) and gene expression analysis using the real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results. SEM revealed bony tissue attachment for both control and test implants. Real-time RT-PCR analysis showed that the expression of osteoblast markers RUNX-2, osteocalcin, alkaline phosphatase, and collagen I was higher (1.62-fold, 1.53-fold, 1.97-fold, and 1.04-fold, resp.) for the implants modified by laminin-1 relative to the control. All osteoclast markers investigated in the study presented higher expression on the test implants than controls as follows: tartrate-resistant acid phosphatase (1.67-fold), calcitonin receptor (1.35-fold), and ATPase (1.25-fold). The test implants demonstrated higher expression of inflammatory markers interleukin-10 (1.53-fold) and tumour necrosis factor-α (1.61-fold) relative to controls. Conclusion. The protein-doped surface showed higher gene expression of typical genes involved in the osseointegration cascade than the control surface. en_US
dc.format.extent 9
dc.language.iso eng en_US
dc.publisher Hindawi en_US
dc.subject.classification Medicine en_US
dc.title The effect of laminin-1-doped nanoroughened implant surfaces : gene expression and morphological evaluation en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Odontology
dc.identifier.doi 10.1155/2012/305638 en_US
dc.subject.srsc Research Subject Categories::ODONTOLOGY en_US
dc.relation.ispartofpublication International Journal of Biomaterials;
dc.relation.ispartofpublicationvolume 2012 en_US
dc.format.artNo 305638
mahlocal.rights.oaType gold
mahlocal.rights.oaType green
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