The objective of this study was to compare dental student’s results at an Objective Structured Clinical Examination (OSCE) with their self-assessed results. These results were compared depending on gender. There are several studies published comparing gender differences in self-assessment in theoretical written exams. However there is a lack of knowledge regarding clinical examinations. At theoretical examinations it has been found that men tend to over-estimate their performance more frequently than women do. Whether these differences in self-assessment exist because men tend to over-estimate themselves, women tend to under-estimate themselves, a combination of both or the self-assessment is consistent with the result at the examination vary between different studies. All students (35 women, 16 men) at the Faculty of Odontology, Malmö University (sixth semester) were examined with an OSCE with 13 stations. The student’s real results at OSCE were compared with their self-assessed results at each station. The assessments were compared regarding gender. At most of the 13 stations there were no significant differences in over- and under-estimation regarding women and men. It was more difficult for men to assess their performance regarding impression with alginate, this difference was statistically significant. These misjudgments were mainly caused by over-estimation. There were no results indicating that women under-estimate their performance at greater extent than men. As opposed to studies where gender differences concerning self-assessment in theoretical examinations are studied, there were no significant differences in over- and under-estimation between genders in clinical examination evaluated in the present study.