Mobilization of Regulatory T Cells in Response to Carotid Injury Does Not Influence Subsequent Neointima Formation

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Mobilization of Regulatory T Cells in Response to Carotid Injury Does Not Influence Subsequent Neointima Formation

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dc.contributor.author Saxena, Amit
dc.contributor.author Björkbacka, Harry
dc.contributor.author Ström, Åsa
dc.contributor.author Rattik, Sara
dc.contributor.author Berg, Katarina E.
dc.contributor.author Gomez, Maria F.
dc.contributor.author Nordin Fredrikson, Gunilla
dc.contributor.author Nilsson, Jan
dc.contributor.author Hultgårdh-Nilsson, Anna
dc.date.accessioned 2013-10-08T08:28:43Z
dc.date.available 2013-10-08T08:28:43Z
dc.date.issued 2012 en_US
dc.identifier.issn 1932-6203 en_US
dc.identifier.uri http://hdl.handle.net/2043/15970
dc.description.abstract Methods and Results A non-obstructive collar was introduced to inflict carotid artery injury in mice and subsequent activation of immune cells in draining lymph nodes and spleen were studied by flow cytometry. Carotid artery injury of wild type mice was associated with mobilization of both Th1 type CD4+IFNγ+ and regulatory CD4+CD25+FoxP3+ T cells in draining lymph nodes. Studies using FoxP3-green fluorescent protein (GFP) transgenic C57/Bl6 mice demonstrated scattered presence of regulatory T cells in the adventitial tissue of injured arteries as well as a massive emigration of regulatory T cells from the spleen in response to carotid injury. However, deletion of antigen presentation to CD4+ T cells (H20 mice), as well as deletion of regulatory T cells (through treatment with blocking anti-CD25 antibodies), did not affect neointima formation. Also deletion of antigen presentation to CD8+ T cells (Tap10 mice) was without effect on carotid collar-induced neointima formation. Conclusion The results demonstrate that carotid artery injury is associated with mobilization of regulatory T cells. Depletion of regulatory T cells does not, however, influence the subsequent repair processes leading to the formation of a neointima. The results also demonstrate that lack of CD8+ T cells does not influence neointima formation in presence of functional CD4+ T cells and B cells. en_US
dc.format.extent 11
dc.language.iso eng en_US
dc.publisher Public Library of Science en_US
dc.subject.classification Medicine en_US
dc.title Mobilization of Regulatory T Cells in Response to Carotid Injury Does Not Influence Subsequent Neointima Formation en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Health and Society en_US
dc.contributor.department Malmö University. Biomedical Sciences (BMV) en_US
dc.identifier.doi 10.1371/journal.pone.0051556 en_US
dc.subject.srsc Research Subject Categories::MEDICINE en_US
dc.relation.ispartofpublication PLOS One;7
dc.relation.ispartofpublicationvolume 12 en_US
dc.format.artNo e51556
mahlocal.rights.oaType gold
mahlocal.rights.oaType green
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