Gingival tissue transcriptomes in experimental gingivitis.

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Gingival tissue transcriptomes in experimental gingivitis.

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dc.contributor.author Ramberg, Per sv
dc.contributor.author Demmer, Ryan T sv
dc.contributor.author Kebschull, Moritz sv
dc.contributor.author Dahlén, Gunnar sv
dc.contributor.author Papapanou, Panos N. sv
dc.contributor.author Jönsson, Daniel en_US
dc.date.accessioned 2013-10-09T05:17:23Z
dc.date.available 2013-10-09T05:17:23Z
dc.date.issued 2011 en_US
dc.identifier.issn 0303-6979 en_US
dc.identifier.uri http://hdl.handle.net/2043/15980
dc.description.abstract AIMS: We investigated the sequential gene expression in the gingiva during the induction and resolution of experimental gingivitis. MATERIAL AND METHODS: Twenty periodontally and systemically healthy non-smoking volunteers participated in a 3-week experimental gingivitis protocol, followed by debridement and 2-week regular plaque control. We recorded clinical indices and harvested gingival tissue samples from four interproximal palatal sites in half of the participants at baseline, Day 7, Day 14 and Day 21 (the "induction phase"), and at Day 21, Day 25, Day 30 and Day 35 in the other half (the "resolution phase"). RNA was extracted, amplified, reversed transcribed, amplified, labelled and hybridized using Affymetrix Human Genome U133Plus2.0 microarrays. Paired t-tests compared gene expression changes between consecutive time points. Gene ontology analyses summarized the expression patterns into biologically relevant categories. RESULTS: The median gingival index was 0 at baseline, 2 at Day 21 and 1 at Day 35. Differential gene regulation peaked during the third week of induction and the first 4 days of resolution. Leucocyte transmigration, cell adhesion and antigen processing/presentation were the top differentially regulated pathways. CONCLUSIONS: Transcriptomic studies enhance our understanding of the pathobiology of the reversible inflammatory gingival lesion and provide a detailed account of the dynamic tissue responses during the induction and resolution of experimental gingivitis. en_US
dc.format.extent 13
dc.language.iso eng en_US
dc.publisher Wiley en_US
dc.subject Experimental gingivitis en_US
dc.subject microarray en_US
dc.subject gene expression en_US
dc.subject tissue response en_US
dc.subject.classification Medicine en_US
dc.title Gingival tissue transcriptomes in experimental gingivitis. en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Odontology
dc.identifier.doi 10.1111/j.1600-051X.2011.01719.x en_US
dc.subject.srsc Research Subject Categories::ODONTOLOGY en_US
dc.identifier.url http://europepmc.org/abstract/MED/21501207 en_US
dc.relation.ispartofpublication Journal of Clinical Periodontology;38
dc.relation.ispartofpublicationvolume 7 en_US
dc.format.ePage 611
dc.format.sPage 599
mahlocal.rights.oaType green
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