Label-free quantitative proteomics reveals differentially regulated proteins in experimental gingivitis

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Label-free quantitative proteomics reveals differentially regulated proteins in experimental gingivitis

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dc.contributor.author Bostanci, Nagihan
dc.contributor.author Ramberg, Per
dc.contributor.author Wahlander, Åsa
dc.contributor.author Grossman, Jonas
dc.contributor.author Jönsson, Daniel
dc.contributor.author Barnes, Virginia Monsul
dc.contributor.author Papapanou, Panos
dc.date.accessioned 2013-10-09T05:43:58Z
dc.date.available 2013-10-09T05:43:58Z
dc.date.issued 2013 en_US
dc.identifier.issn 1535-3893 en_US
dc.identifier.uri http://hdl.handle.net/2043/15983
dc.description.abstract We investigated the sequential protein expression in gingival crevicular fluid samples during the induction (I) and resolution (R) of experimental gingivitis. Periodontally and systemically healthy volunteers (n = 20) participated in a three-week experimental gingivitis protocol, followed by debridement and two weeks of regular plaque control. Gingival crevicular fluid (GCF) samples were collected at baseline, Day 7, 14, and 21 (induction; I-phase), and at Day 21, 25, 30, and 35 (resolution; R-phase). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for label-free quantitative proteomics was applied. A total of 287 proteins were identified including 254 human, 14 bacterial, 12 fungal, and 7 yeast proteins. Ontology analysis revealed proteins primarily involved in cytoskeletal rearrangements, immune response, antimicrobial function, protein degradation, and DNA binding. There was considerable variation in the number of proteins identified, both among subjects and within subjects across time points. After pooling of samples between subjects at each time point, the levels of 59 proteins in the I-phase and 73 proteins in the R-phase were quantified longitudinally. Our data demonstrate that LC-MS/MS label-free quantitative proteomics is valuable in the assessment of the protein content of the GCF and can facilitate a better understanding of the molecular mechanisms involved in the induction and resolution of plaque-induced gingival inflammation in humans. en_US
dc.format.extent 22
dc.language.iso eng en_US
dc.publisher American Chemical Society en_US
dc.subject gingival crevicular fluid en_US
dc.subject inflammation en_US
dc.subject biomarker en_US
dc.subject periodontal disease en_US
dc.subject pathogenesis en_US
dc.subject.classification Medicine en_US
dc.title Label-free quantitative proteomics reveals differentially regulated proteins in experimental gingivitis en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Odontology
dc.identifier.doi 10.1021/pr300761e en_US
dc.subject.srsc Research Subject Categories::ODONTOLOGY en_US
dc.relation.ispartofpublication Journal of Proteome Research;12
dc.relation.ispartofpublicationvolume 2 en_US
dc.format.ePage 678
dc.format.sPage 657
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