Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces

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Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces

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dc.contributor.author Sotres, Javier
dc.contributor.author Barrantes, Alejandro
dc.contributor.author Lindh, Liselott
dc.contributor.author Arnebrant, Thomas
dc.date.accessioned 2013-12-10T09:31:32Z
dc.date.available 2013-12-10T09:31:32Z
dc.date.issued 2014 en_US
dc.identifier.citation 98-110 en_US
dc.identifier.issn 0008-6568 en_US
dc.identifier.uri http://hdl.handle.net/2043/16248
dc.description.abstract We show in this work how systems formed by phosphoproteins on calcium phosphate surfaces can be directly characterized, in real time, in liquid medium, without the need for elution or labeling. Specifically, we show how this is possible by applying three different techniques: ellipsometry, quartz crystal microbalance with dissipation, and atomic force microscopy-based friction force spectroscopy. We apply these techniques to study two different model systems, i.e. those formed upon the adsorption of two model phosphoproteins (κ- and β-casein) on hydroxyapatite (HA) surfaces. Information on the kinetics of adsorption, surface excess, viscoelasticity, water content, thickness of the layers, and protein-surface interaction is provided. Results indicate that both phosphoproteins form homogeneous elastic highly hydrated monolayers on the HA surfaces, the strength of β-casein layers being higher by approximately a factor of 4. Based on the experimental results, models for the conformation of κ- and β-casein molecules adsorbed on HA surfaces are proposed. en_US
dc.language.iso eng en_US
dc.publisher Karger en_US
dc.subject Phosphoprotein en_US
dc.subject casein en_US
dc.subject hydroxyapatite en_US
dc.subject atomic force microscopy en_US
dc.subject friction force spectroscopy en_US
dc.subject ellipsometry en_US
dc.subject quartz crystal microbalance with dissipation en_US
dc.subject.classification Sciences en_US
dc.title Strategies for a Direct Characterization of Phosphoproteins on Hydroxyapatite Surfaces en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Health and Society en
dc.contributor.department Malmö University. Faculty of Odontology en
dc.identifier.doi 10.1159/000351871 en_US
dc.subject.srsc Research Subject Categories::NATURAL SCIENCES en_US
dc.relation.ispartofpublication Caries Research;2
dc.relation.ispartofpublicationvolume 48 en_US
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