Controlled release of Clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects

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Controlled release of Clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects

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dc.contributor.author Alenezi, Ali en_US
dc.contributor.author Naito, Yoshihito en_US
dc.contributor.author Terukina, Takayuki en_US
dc.contributor.author Prananingrum, Widyasri en_US
dc.contributor.author Jinno, Yohei en_US
dc.contributor.author Tagami, Tatsuaki en_US
dc.contributor.author Ozeki, Tetsuya en_US
dc.contributor.author Galli, Silvia en_US
dc.contributor.author Jimbo, Ryo en_US
dc.date.accessioned 2017-06-01T09:00:27Z
dc.date.available 2017-06-01T09:00:27Z
dc.date.issued 2017 en_US
dc.identifier.issn 1552-4981 en_US
dc.identifier.uri http://hdl.handle.net/2043/22685
dc.description.abstract Purpose: To evaluate the controlled release effect of Clarithromycin loaded in PLGA microspheres in a rabbit calvaria defect model. Methods: Clarithromycin-loaded PLGA microspheres (MSPs) were formulated by modified O/W single emulsion/solvent evaporation method. After characterization, in vivo animal experiment was conducted. Four critical size bone defects were created in the calvaria of New Zealand White rabbits (n=21, n=7/time point). The bone defects were randomly designated to 4 groups: Group 1: No augmentation (sham), Group 2: beta-Tricalcium phosphate (β-TCP), Group 3: beta-Tricalcium phosphate (β-TCP) with 0.12 µg clarithromycin, and Group 4: beta-Tricalcium phosphate (β-TCP) with 6.12 µg PLGA microspheres (loaded with 0.12 µg clarithromycin). After 2, 4 and 12 weeks of healing, the levels of bone regeneration were evaluated using micro- computed tomography and histology. Results: The average size of the PLGA microspheres was 26.38 μm that showed 94% encapsulation efficacy with clarithromycin. Clarithromycin release from PLGA microspheres revealed sustained release for around 4 weeks with approximately 50% release of clarithromycin during the first week. In the histological analysis, new bone formation was evident at 2 and 4 weeks of healing in all groups and bone formation increased as a function of healing time in vivo. At 12 weeks, Group 4 showed significantly higher amount of newly formed bone compared to Group 1 (p=0,002). Moreover, during the micro CT exam, Group 4 expressed significantly higher bone formation compared to Group 1 at all time points tested (p=0.00, 0.014, and 0.002 in 2, 4, and 12 weeks, respectively). Conclusions: PLGA microspheres demonstrated initial burst release of clarithromycin followed by a sustained release profile. The in vivo findings showed that β-TCP with clarithromycin-loaded microspheres can enhance bone formation in bone defects. en_US
dc.format.extent 8
dc.language.iso eng en_US
dc.publisher Wiley en_US
dc.subject microspheres en_US
dc.subject drug delivery system en_US
dc.subject sustained release en_US
dc.subject clarithromycin en_US
dc.subject.classification Medicine en_US
dc.title Controlled release of Clarithromycin from PLGA microspheres enhances bone regeneration in rabbit calvaria defects en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Odontology
dc.identifier.doi 10.1002/jbm.b.33844 en_US
dc.subject.srsc Research Subject Categories::ODONTOLOGY en_US
dc.identifier.url http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33844/full en_US
dc.relation.ispartofpublication Journal of Biomedical Materials Research Part B : Applied Biomaterials;1
dc.relation.ispartofpublicationvolume 106 en_US
dc.description.authorversion Yes en_US
dc.contributor.centre Malmö University. Biofilms - Research Center for Biointerfaces en_US
dc.format.ePage 208
dc.format.sPage 201
mahlocal.rights.oaType green
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