Electrical activity of cellobiose dehydrogenase adsorbed on thiols : Influence of charge and hydrophobicity

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Electrical activity of cellobiose dehydrogenase adsorbed on thiols : Influence of charge and hydrophobicity

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Publication Article, peer reviewed scientific
Title Electrical activity of cellobiose dehydrogenase adsorbed on thiols : Influence of charge and hydrophobicity
Author Lamberg, P. ; Hamit-Eminovski, J. ; Toscano, M. D. ; Eicher-Lorka, O. ; Niaura, G. ; Arnebrant, Thomas ; Shleev, Sergey ; Ruzgas, Tautgirdas
Date 2017
English abstract
The interface between protein and material surface is of great research interest in applications varying from implants, tissue engineering to bioelectronics. Maintaining functionality of bioelements depends greatly on the immobilization process. In the present study direct electron transfer of cellobiose dehydrogenase from Humicola insolens (HiCDH), adsorbed on four different self-assembled monolayers (SAMs) formed by 5-6 chain length carbon thiols varying in terminal group structure was investigated. By using a combination of quartz crystal micro balance with dissipation, ellipsometry and electrochemistry the formation and function of the HiCDH film was studied. It was found that the presence of charged pyridinium groups was needed to successfully establish direct electron contact between the enzyme and electrode. SAMs formed from hydrophilic charged thiols achieved nearly two times higher current densities compared to hydrophobic charged thiols. Additionally, the results also indicated proportionality between HiCDH catalytic constant and water content of the enzyme film. Enzyme films on charged pyridine thiols had smaller variations in water content and viscoelastic properties than films adsorbed on the more hydrophobic thiols. This work highlights several perspectives on the underlying factors affecting performance of immobilized HiCDH. (C) 2017 Elsevier B.V. All rights reserved.
DOI https://doi.org/10.1016/j.bioelechem.2017.02.001 (link to publisher's fulltext.)
Publisher Elsevier
Host/Issue Bioelectrochemistry;
Volume 115
ISSN 1567-5394
Language eng (iso)
Subject Cellobiose dehydrogenase
Direct electron transfer
Self-assembled monolayers
Surface charge
Hydrophobicity
Thiol
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/23586 Permalink to this page
Link to publication in DiVA Find this research publication in DiVA (n/a for student publ.)
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