Reversible Self-Assembled Monolayers (rSAMs) : Adaptable Surfaces for Enhanced Multivalent Interactions and Ultrasensitive Virus Detection

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Reversible Self-Assembled Monolayers (rSAMs) : Adaptable Surfaces for Enhanced Multivalent Interactions and Ultrasensitive Virus Detection

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Publication Article, peer reviewed scientific
Title Reversible Self-Assembled Monolayers (rSAMs) : Adaptable Surfaces for Enhanced Multivalent Interactions and Ultrasensitive Virus Detection
Author Yeung, Sing Yee ; Mucha, Annabell ; Deshmukh, Ravindra ; Boutrus, Malak ; Arnebrant, Thomas ; Sellergren, Börje
Research Centre Biofilms - Research Center for Biointerfaces
Date 2017
English abstract
We report on the design of pH-switchable monolayers allowing a reversible and ordered introduction of affinity reagents on sensor surfaces. The principal layer building blocks consist of α-(4-amidinophenoxy)alkanes decorated at the ω-position with affinity ligands. These spontaneously self-assemble on top of carboxylic acid terminated SAMs to form reversible homo or mixed monolayers (rSAMs) that are tunable with respect to the nature of the head group, layer order and stability while featuring pH responsiveness and the dynamic nature of noncovalent build assemblies. We show that this results in a range of unique biosensor features. As a first example a sialic acid rSAM featuring strong lectin affinity is here used to sense hemagglutinin and influenza virus (H5N1) at the pM and fM level by in situ ellipsometry in a fully reversible fashion. We believe that the rSAM concept will find widespread use in surface chemistry and overall for boosting sensitivity in affinity biosensors.
DOI https://doi.org/10.1021/acscentsci.7b00412 (link to publisher's fulltext.)
Link http://pubs.acs.org/doi/10.1021/acscentsci.7b00412 .Icon
Publisher American Chemical Society
Host/Issue ACS Central Science;11
Volume 3
ISSN 2374-7951
Pages 10
Page 1198-1207
Language eng (iso)
Subject Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/24188 Permalink to this page
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