Human lipoproteins at model cell membranes : Role of the lipoprotein class on lipid dynamics

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Human lipoproteins at model cell membranes : Role of the lipoprotein class on lipid dynamics

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Publication Article, peer reviewed scientific
Title Human lipoproteins at model cell membranes : Role of the lipoprotein class on lipid dynamics
Author Browning, T. K. ; Lind, Tania Kjellerup ; Maric, Selma ; Malekkhaiat-Häffner, S. ; Fredrikson, G. N. ; Bengtsson, E. ; Malmsten, M. ; Cárdenas, Marité
Research Centre Biofilms - Research Center for Biointerfaces
Date 2017
English abstract
High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the ‘good’ cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the ‘bad’ cholesterol, depositing lipid material into the vascular wall.
Link https://www.nature.com/articles/s41598-017-07505-0 .Icon
Publisher Nature Publishing Group
Host/Issue Scientific Reports;
Volume 7
ISSN 2045-2322
Language eng (iso)
Subject Biophysical chemistry
Characterization and analytical techniques
Diagnostic markers
Membrane biophysics
Membrane structure and assembly
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/24203 Permalink to this page
Link to publication in DiVA Find this research publication in DiVA (n/a for student publ.)
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