The lantibiotic gallidermin acts bactericidal against Staphylococcus epidermidis and Staphylococcus aureus and antagonizes the bacteria‐induced proinflammatory responses in dermal fibroblasts

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The lantibiotic gallidermin acts bactericidal against Staphylococcus epidermidis and Staphylococcus aureus and antagonizes the bacteria‐induced proinflammatory responses in dermal fibroblasts

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Publication Article, peer reviewed scientific
Title The lantibiotic gallidermin acts bactericidal against Staphylococcus epidermidis and Staphylococcus aureus and antagonizes the bacteria‐induced proinflammatory responses in dermal fibroblasts
Author Bengtsson, Torbjörn ; Lönn, Johanna ; Khalaf, Hazem ; Eleonor, Palm
Research Centre Biofilms - Research Center for Biointerfaces
Date 2018
English abstract
Antimicrobial resistance needs to be tackled from new angles, and antimicrobial peptides could be future candidates for combating bacterial infections. This study aims to investigate in vitro the bactericidal effects of the lantibiotic gallidermin on Staphylococcus epidermidis and Staphylococcus aureus, possible cytotoxic effects and its impact on host‐microbe interactions. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of gallidermin were determined, and cytotoxicity and proinflammatory effects of gallidermin on fibroblasts, red blood cells (RBCs) and in whole blood were investigated. Both MIC and MBC for all four tested strains of S. epidermidis was 6.25 μg/ml. Both MIC and MBC for methicillin‐sensitive S. aureus was 12.5 μg/ml and for methicillin‐resistant S. aureus (MRSA) 1.56 μg/ml. Gallidermin displayed no cytotoxic effects on fibroblasts, only a high dose of gallidermin induced low levels of CXCL8 and interleukin‐6. Gallidermin hemolyzed less than 1% of human RBCs, and did not induce reactive oxygen species production or cell aggregation in whole blood. In cell culture, gallidermin inhibited the cytotoxic effects of the bacteria and totally suppressed the bacteria‐induced release of CXCL8 and interleukin‐6 from fibroblasts. We demonstrate that gallidermin, expressing low cell cytotoxicity, is a promising candidate for treating bacterial infections caused by S. epidermidis and S. aureus, especially MRSA.
DOI https://doi.org/10.1002/mbo3.606 (link to publisher's fulltext.)
Link https://onlinelibrary.wiley.com/doi/full/10.1002/mbo3.606 .Icon
Publisher Wiley
Host/Issue MicrobiologyOpen;6
Volume 7
ISSN 2045-8827
Language eng (iso)
Subject Medicine
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/24756 Permalink to this page
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