HLA DR-DQ genotyping by capillary electrophoresis for risk assessment for celiac disease.

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HLA DR-DQ genotyping by capillary electrophoresis for risk assessment for celiac disease.

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Publication BookChapter
Title HLA DR-DQ genotyping by capillary electrophoresis for risk assessment for celiac disease.
Author Lavant, Ewa ; Carlson, Joyce
Date 2013
English abstract
The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.
DOI https://doi.org/10.1007/978-1-62703-029-8_26 (link to publisher's fulltext.)
Publisher Humana Press
Host/Issue Clinical Applications of Capillary Electrophoresis
Series/Issue Methods in Molecular Biology; 919
ISSN 1064-3745
ISBN 978-1-62703-028-1
978-1-62703-029-8
Pages 11
Page 297-307
Language eng (iso)
Subject genetics immunology
immunology
methods
Genetic Predisposition to Disease
Medicine
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/27414 Permalink to this page
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