Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations

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Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations

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Publication Article, peer reviewed scientific
Title Nanometer Scale Titanium Surface Texturing Are Detected by Signaling Pathways Involving Transient FAK and Src Activations
Author Zambuzzi, Willian F. ; Bonfante, Estevam A. ; Hayashi, Mariko ; Andersson, Martin ; Alves, Gutemberg ; Takamori, Esther R. ; Beltrao, Paulo J. ; Coelho, Paulo G. ; Granjeiro, Jose M. ; Jimbo, Ryo
Date 2014
English abstract
Background: It is known that physico/chemical alterations on biomaterial surfaces have the capability to modulate cellular behavior, affecting early tissue repair. Such surface modifications are aimed to improve early healing response and, clinically, offer the possibility to shorten the time from implant placement to functional loading. Since FAK and Src are intracellular proteins able to predict the quality of osteoblast adhesion, this study evaluated the osteoblast behavior in response to nanometer scale titanium surface texturing by monitoring FAK and Src phosphorylations. Methodology: Four engineered titanium surfaces were used for the study: machined (M), dual acid-etched (DAA), resorbable media microblasted and acid-etched (MBAA), and acid-etch microblasted (AAMB). Surfaces were characterized by scanning electron microscopy, interferometry, atomic force microscopy, x-ray photoelectron spectroscopy and energy dispersive X-ray spectroscopy. Thereafter, those 4 samples were used to evaluate their cytotoxicity and interference on FAK and Src phosphorylations. Both Src and FAK were investigated by using specific antibody against specific phosphorylation sites. Principal Findings: The results showed that both FAK and Src activations were differently modulated as a function of titanium surfaces physico/chemical configuration and protein adsorption. Conclusions: It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces.
DOI https://doi.org/10.1371/journal.pone.0095662 (link to publisher's fulltext.)
Link https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095662 .Icon
Publisher Public Library of Science
Host/Issue PloS One;7
Volume 9
ISSN 1932-6203
Language eng (iso)
Subject Multidisciplinary Sciences
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/27438 Permalink to this page
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