Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor : Complementary Match of Supramolecular and Dynamic Chemistry

DSpace Repository

Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor : Complementary Match of Supramolecular and Dynamic Chemistry

Details

Files for download

Find Full text There are no files associated with this item..

Overview of item record
Publication Article, peer reviewed scientific
Title Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor : Complementary Match of Supramolecular and Dynamic Chemistry
Author Bergdahl, Gizem Ertürk ; Akhoundian, Maedeh ; Lueg-Althoff, Kyra ; Yeung, Sing Yee ; Hedström, Martin ; Schrader, Thomas ; Matiasson, Bo ; Sellergren, Börje ; Shinde, Sudhirkumar
Research Centre Biofilms - Research Center for Biointerfaces
Date 2019
English abstract
Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for Trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the strong interaction of bisphosphonate with lysine/arginine residues on the surface of Trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition.
DOI https://doi.org/10.1039/C8NJ05238G (link to publisher's fulltext.)
Publisher Royal Society of Chemistry
Host/Issue New Journal of Chemistry;2
Volume 43
ISSN 1144-0546
Language eng (iso)
Subject Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/27974 Permalink to this page
Facebook

This item appears in the following Collection(s)

Details

Search


Browse

My Account

Statistics