
Utskrift från Malmö universitet - mau.se
Utskrift från Malmö universitet - mau.se
Publication | Article, peer reviewed scientific |
Title | Bisphosphonate Ligand Mediated Ultrasensitive Capacitive Protein Sensor : Complementary Match of Supramolecular and Dynamic Chemistry |
Author | ; ; ; ; ; ; ; ; |
Research Centre | Biofilms - Research Center for Biointerfaces |
Date | 2019 |
English abstract | |
Modern healthcare demands rapid and accurate detection of proteins/enzymes at the ultratrace level. Herein we present a molecularly imprinted capacitive sensor for Trypsin, developed by microcontact imprinting. High affinity and selectivity was achieved by doping the prepolymerization mixture with a stoichiometric amount of methacrylamide-based bisphosphonate (BP) monomer. Taking advantage of the strong interaction of bisphosphonate with lysine/arginine residues on the surface of Trypsin, we have constructed a powerful polymeric sensor. The BP based sensor has the ability to recognize trypsin over other arginine-rich proteins, even in high ionic strength buffers with a sub-picomolar detection limit (pM). We believe that the combination of supramolecular chemistry, molecular imprinting and advanced instrumentation has a potential for future drug development and diagnostics that extends beyond biomolecular recognition. | |
DOI | https://doi.org/10.1039/C8NJ05238G (link to publisher's fulltext.) |
Publisher | Royal Society of Chemistry |
Host/Issue | New Journal of Chemistry;2 |
Volume | 43 |
ISSN | 1144-0546 |
Language | eng (iso) |
Subject | Sciences Research Subject Categories::NATURAL SCIENCES |
Handle | http://hdl.handle.net/2043/27974 Permalink to this page |
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