Protein expression by planktonic and biofilm cells of Streptococcus mutans

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Protein expression by planktonic and biofilm cells of Streptococcus mutans

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Publication Article, peer reviewed scientific
Title Protein expression by planktonic and biofilm cells of Streptococcus mutans
Author Svensäter, Gunnel ; Welin, J ; Wilkins, J C ; Beighton, D ; Hamilton, I R
Research Centre Biofilms - Research Center for Biointerfaces
Date 2001
English abstract
Streptococcus mutans, a major causal agent of dental caries, functions in nature as a component of a biofilm on teeth (dental plaque) and yet very little information is available on the physiology of the organism in such surface-associated communities. As a consequence, we undertook to examine the synthesis of proteins by planktonic and biofilm cells growing in a biofilm chemostat at pH 7.5 at a dilution rate of 0.1 h(-1) (mean generation time=7 h). Cells were incubated with (14)C-labelled amino acids, the proteins extracted and separated by two-dimensional electrophoresis followed by autoradiography and computer-assisted image analysis. Of 694 proteins analysed, 57 proteins were enhanced 1.3-fold or greater in biofilm cells compared to planktonic cells with 13 only expressed in sessile cells. Diminished protein expression was observed with 78 proteins, nine of which were not expressed in biofilm cells. The identification of enhanced and diminished proteins by mass spectrometry and computer-assisted protein sequence analysis revealed that, in general, glycolytic enzymes involved in acid formation were repressed in biofilm cells, while biosynthetic processes were enhanced. The results show that biofilm cells possess novel proteins, of as yet unknown function, that are not present in planktonic cells.
DOI https://doi.org/10.1111/j.1574-6968.2001.tb10937.x (link to publisher's fulltext.)
Link https://doi.org/10.1111/j.1574-6968.2001.tb10937.x .Icon
Publisher Elsevier
Host/Issue FEMS microbiology letters;1
Volume 205
ISSN 0378-1097
Language eng (iso)
Subject Medicine
Animals
Bacterial Proteins
Biofilms
Culture Media
Dental Caries
Electrophoresis, Gel, Two-Dimensional
Humans
Image Processing, Computer-Assisted
Plankton
Streptococcus mutans
Research Subject Categories::ODONTOLOGY
Handle http://hdl.handle.net/2043/28589 Permalink to this page
Link to publication in DiVA Find this research publication in DiVA.
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