Synergism by individual macronutrients explains the marked early GLP-1 and islet hormone responses to mixed meal challenge in mice

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Synergism by individual macronutrients explains the marked early GLP-1 and islet hormone responses to mixed meal challenge in mice

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Publication Article, peer reviewed scientific
Title Synergism by individual macronutrients explains the marked early GLP-1 and islet hormone responses to mixed meal challenge in mice
Author Ahlkvist, L ; Vikman, Jenny ; Pacini, G ; Ahrén, B
Date 2012
English abstract
Apart from glucose, proteins and lipids also stimulate incretin and islet hormone secretion. However, the glucoregulatory effect of macronutrients in combination is poorly understood. We therefore developed an oral mixed meal model in mice to 1) explore the glucagon-like peptide-1 (GLP-1) and islet hormone responses to mixed meal versus isocaloric glucose, and 2) characterize the relative contribution of individual macronutrients to these responses. Anesthetized C57BL/6J female mice were orally gavaged with 1) a mixed meal (0.285 kcal; glucose, whey protein and peanut oil; 60/20/20% kcal) versus an isocaloric glucose load (0.285 kcal), and 2) a mixed meal (0.285 kcal) versus glucose, whey protein or peanut oil administered individually in their mixed meal caloric quantity, i.e., 0.171, 0.055 and 0.055 kcal, respectively. Plasma was analyzed for glucose, insulin and intact GLP-1 before and during oral challenges. Plasma glucose was lower after mixed meal versus after isocaloric glucose ingestion. In spite of this, the peak insulin response (P=0.02), the peak intact GLP-1 levels (P=0.006) and the estimated β-cell function (P=0.005) were higher. Furthermore, the peak insulin (P=0.004) and intact GLP-1 (P=0.006) levels were higher after mixed meal ingestion than the sum of responses to individual macronutrients. Compared to glucose alone, we conclude that there is a marked early insulin response to mixed meal ingestion, which emanates from a synergistic, rather than an additive, effect of the individual macronutrients in the mixed meal and is in part likely caused by increased levels of GLP-1.
DOI https://doi.org/10.1016/j.regpep.2012.06.004 (link to publisher's fulltext.)
Host/Issue Regulatory peptides;1-3
Volume 178
ISSN 1873-1686
Language eng (iso)
Subject Macronutrients
Insulin
GLP-1
Incretin
Mixed meal
Medicine
Administration, Oral
Animals
Area Under Curve
Blood Glucose
Diet
Female
Glucagon-Like Peptide 1
Glucose
Glucose Tolerance Test
Incretins
Insulin
Insulin Resistance
Insulin Secretion
Islets of Langerhans
Linear Models
Mice
Mice, Inbred C57BL
Milk Proteins
Peanut Oil
Plant Oils
Whey Proteins
Research Subject Categories::MEDICINE
Handle http://hdl.handle.net/2043/28769 Permalink to this page
Link to publication in DiVA Find this research publication in DiVA.
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