The Effect of UVB Irradiation and Oxidative Stress on the Skin Barrier : A New Method to Evaluate Sun Protection Factor Based on Electrical Impedance Spectroscopy

DSpace Repository

The Effect of UVB Irradiation and Oxidative Stress on the Skin Barrier : A New Method to Evaluate Sun Protection Factor Based on Electrical Impedance Spectroscopy

Overview

Detailed record

dc.contributor.author Hernández, Aura Rocio sv
dc.contributor.author Vallejo, Bibiana sv
dc.contributor.author Ruzgas, Tautgirdas en_US
dc.contributor.author Björklund, Sebastian en_US
dc.date.accessioned 2019-08-13T10:27:07Z
dc.date.available 2019-08-13T10:27:07Z
dc.date.issued 2019 en_US
dc.identifier.issn 1424-8220 en_US
dc.identifier.other 31126113
dc.identifier.uri http://hdl.handle.net/2043/29612
dc.description.abstract Sunlight is vital for several biochemical processes of the skin organ. However, acute or chronic exposure to ultraviolet radiation (UVR) has several harmful effects on the skin structure and function, especially in the case of the failing function of antioxidative enzymes, which may lead to substantial tissue damage due to the increased presence of reactive oxygen species (ROS). The aim of this work was to investigate the combined effect of ultraviolet B (UVB) irradiation and oxidative stress on the skin barrier integrity. For this, we employed electrical impedance spectroscopy (EIS) to characterize changes of the electrical properties of excised pig skin membranes after various exposure conditions of UVB irradiation, oxidative stress, and the inhibition of antioxidative enzymatic processes. The oxidative stress was regulated by adding hydrogen peroxide (HO) as a source of ROS, while sodium azide (NaN) was used as an inhibitor of the antioxidative enzyme catalase, which is naturally present throughout the epidermis. By screening for the combined effect of UVB and oxidative stress on the skin membrane electrical properties, we developed a new protocol for evaluating these parameters in a simple in vitro setup. Strikingly, the results show that exposure to extreme UVB irradiation does not affect the skin membrane resistance, implying that the skin barrier remains macroscopically intact. Likewise, exposure to only oxidative stress conditions, without UVB irradiation, does not affect the skin membrane resistance. In contrast to these observations, the combination of UVB irradiation and oxidative stress conditions results in a drastic decrease of the skin membrane resistance, indicating that the integrity of the skin barrier is compromised. Further, the skin membrane effective capacitance remained more or less unaffected by UVB exposure, irrespective of simultaneous exposure of oxidative stress. The EIS results were concluded to be associated with clear signs of macroscopic tissue damage of the epidermis as visualized with microscopy after exposure to UVB irradiation under oxidative stress conditions. Finally, the novel methodology was tested by performing an assessment of cosmetic sunscreen formulations with varying sun protection factor (SPF), with an overall successful outcome, showing good correlation between SPF value and protection capacity in terms of skin resistance change. The results from this study allow for the development of new skin sensors based on EIS for the detection of skin tissue damage from exposure to UVB irradiation and oxidative stress and provide a new, more comprehensive methodology, taking into account both the influence of UVB irradiation and oxidative stress, for in vitro determination of SPF in cosmetic formulations. en_US
dc.format.extent 16 en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.subject UVB irradiation en_US
dc.subject azide en_US
dc.subject catalase en_US
dc.subject cosmetic sunscreen en_US
dc.subject epidermis en_US
dc.subject hydrogen peroxide en_US
dc.subject oxidative stress en_US
dc.subject stratum corneum en_US
dc.subject sun protection factor en_US
dc.subject.classification Sciences en_US
dc.title The Effect of UVB Irradiation and Oxidative Stress on the Skin Barrier : A New Method to Evaluate Sun Protection Factor Based on Electrical Impedance Spectroscopy en_US
dc.type Article, peer reviewed scientific en_US
dc.identifier.doi 10.3390/s19102376 en_US
dc.description.other Sensors (Basel)
dc.subject.srsc Research Subject Categories::NATURAL SCIENCES en_US
dc.relation.ispartofpublication Sensors;10
dc.relation.ispartofpublicationvolume 19 en_US
dc.description.authorversion No en_US
dc.identifier.isiid 000471014500163
dc.contributor.centre Malmö University. Biofilms - Research Center for Biointerfaces en_US
dc.identifier.pmid 31126113
dcterms.identifier.OAurl http://dx.doi.org/10.3390/s19102376 en_US
dc.format.artNo 2376
 Find Full text Files for download
Icon

This item appears in the following Collection(s)

Overview

Search


Browse

My Account

Statistics