New concepts for transdermal delivery of oxygen based on catalase biochemical reactions studied by oxygen electrode amperometry

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New concepts for transdermal delivery of oxygen based on catalase biochemical reactions studied by oxygen electrode amperometry

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Publication Article, peer reviewed scientific
Title New concepts for transdermal delivery of oxygen based on catalase biochemical reactions studied by oxygen electrode amperometry
Author Hernández, Aura Rocio ; Boutonnet, Marine ; Svensson, Birgitta ; Butler, Eile ; Lood, Rolf ; Blom, Kristina ; Vallejo, Bibiana ; Anderson, Chris ; Engblom, Johan ; Ruzgas, Tautgirdas ; Björklund, Sebastian
Research Centre Biofilms - Research Center for Biointerfaces
Date 2019
English abstract
The development of formulation concepts for improved skin tissue oxygenation, including methods for measuring oxygen (O) transport across biological barriers, are important research topics with respect to all processes that are affected by the O concentration, such as radiation therapy in oncology treatments, wound healing, and the general health status of skin. In this work we approach this topic by a novel strategy based on the antioxidative enzyme catalase, which is naturally present in the skin organ where it enables conversion of the reactive oxygen species hydrogen peroxide (HO) into O. We introduce various applications of the skin covered oxygen electrode (SCOE) as an in-vitro tool for studies of catalase activity and function. The SCOE is constructed by placing an excised skin membrane directly on an O electrode and the methodology is based on measurements of the electrical current generated by reduction of O as a function of time (i.e. chronoamperometry). The results confirm that a high amount of native catalase is present in the skin organ, even in the outermost stratum corneum (SC) barrier, and we conclude that excised pig skin (irrespective of freeze-thaw treatment) represents a valid model for ex vivo human skin for studying catalase function by the SCOE setup. The activity of native catalase in skin is sufficient to generate considerable amounts of O by conversion from HO and proof-of-concept is presented for catalase-based transdermal O delivery from topical formulations containing HO. In addition, we show that this concept can be further improved by topical application of external catalase on the skin surface, which enables transdermal O delivery from 50 times lower concentrations of HO. These important results are promising for development of novel topical or transdermal formulations containing low and safe concentrations of HO for skin tissue oxygenation. Further, our results indicate that the O production by catalase, derived from topically applied S. epidermidis (a simple model for skin microbiota) is relatively low as compared to the O produced by the catalase naturally present in skin. Still, the catalase activity derived from S. epidermidis is measurable. Taken together, this work illustrates the benefits and versatility of the SCOE as an in vitro skin research tool and introduces new and promising strategies for transdermal oxygen delivery, with simultaneous detoxification of HO, based on native or topically applied catalase.
DOI https://doi.org/10.1016/j.jconrel.2019.06.001 (link to publisher's fulltext.)
Publisher Elsevier
Host/Issue Journal of Controlled Release;
Volume 306
ISSN 1873-4995
Pages 121-129
Language eng (iso)
Subject Catalase
Epidermis
Hydrogen peroxide
Oxygen electrode
Skin microbiota
Skin tissue oxygenation
Stratum corneum
Topical and transdermal oxygen delivery
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/29616 Permalink to this page
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