Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes

DSpace Repository

Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes

Details

Files for download
Icon
Overview of item record
Publication Article, peer reviewed scientific
Title Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes
Author Maric, Selma ; Lind, Tania Kjellerup ; Raida, Manfred Roman ; Bengtsson, Eva ; Fredrikson, Gunilla Nordin ; Rogers, Sarah ; Moulin, Martine ; Haertlein, Michael ; Forsyth, V. Trevor ; Wenk, Markus R. ; Pomorski, Thomas Guenther ; Arnebrant, Thomas ; Lund, Reidar ; Cardenas, Marite
Date 2019
English abstract
Atherosclerosis is the main killer in the western world. Today's clinical markers include the total level of cholesterol and high-/low-density lipoproteins, which often fails to accurately predict the disease. The relationship between the lipid exchange capacity and lipoprotein structure should explain the extent by which they release or accept lipid cargo and should relate to the risk for developing atherosclerosis. Here, small-angle neutron scattering and tailored deuteration have been used to follow the molecular lipid exchange between human lipoprotein particles and cellular membrane mimics made of natural, "neutron invisible" phosphatidylcholines. We show that lipid exchange occurs via two different processes that include lipid transfer via collision and upon direct particle tethering to the membrane, and that high-density lipoprotein excels at exchanging the human-like unsaturated phosphatidylcholine. By mapping the specific lipid content and level of glycation/oxidation, the mode of action of specific lipoproteins can now be deciphered. This information can prove important for the development of improved diagnostic tools and in the treatment of atherosclerosis.
DOI https://doi.org/10.1038/s41598-019-43713-6 (link to publisher's fulltext.)
Link https://www.nature.com/articles/s41598-019-43713-6 .Icon
Publisher Nature Publishing Group
Host/Issue Scientific Reports;1
Volume 9
ISSN 2045-2322
Language eng (iso)
Subject Multidisciplinary Sciences
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/30138 Permalink to this page
Facebook

This item appears in the following Collection(s)

Details

Search


Browse

My Account

Statistics