Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules

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Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules

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Publication Article, peer reviewed scientific
Title Impact of molecular linker size on physicochemical properties of assembled gold nanoparticle mono-/multi-layers and their applicability for functional binding of biomolecules
Author Krikstolaityte, Vida ; Hamit-Eminovski, Jildiz ; Abariute, Laura ; Niaura, Gediminas ; Meskys, Rolandas ; Arnebrant, Thomas ; Lisak, Grzegorz ; Ruzgas, Tautgirdas
Research Centre Biofilms - Research Center for Biointerfaces
Date 2019
English abstract
In this work the impact of molecular inter-linker size on gold nanoparticle (AuNP) mono-/multilayer structural properties, density and homogeneity has been investigated. These characteristics are of great importance for functional binding of biomolecules. Positively charged high or low molecular weight inter-linkers, poly-L-lysine (PLL) or N-(6-mercapto)hexylpyridinium (MHP), were used to attach negatively charged AuNPs on a planar gold surface as well as to further interlink into a multilayer structure via layer-by-layer deposition. The inter-particle interaction within the assembled AuNP films was adjusted by the ionic strength in the AuNPs dispersions The AuNP layer density and structural/viscoelastic properties were evaluated by the quartz crystal microbalance with dissipation (QCM-D) technique. The validity of the commercial Voigt model, specifically developed for quantitative QCM-D data analysis of homogeneous viscoelastic films, was evaluated by a model independent analysis when comparing the assembled AuNP films with a homogeneous layer of a mucin from bovine submaxillary glands. Both AuNP mono- and multilayers, attached/interlinked via long flexible PLL molecules assembled to denser and more soft/viscous structures compared to those interlinked by short MHP compounds. Thus, PLL-interlinked AuNP mono-/multilayer structures were further investigated as a platform for laccase enzyme functional adsorption via qualitative assessment of bioelectrochemical characteristics of the enzyme. (C) 2019 Elsevier Inc. All rights reserved.
DOI https://doi.org/10.1016/j.jcis.2019.02.048 (link to publisher's fulltext.)
Publisher Elsevier
Host/Issue Journal of Colloid and Interface Science;
Volume 543
ISSN 0021-9797
Language eng (iso)
Subject Poly-L-lysine
N-(6-mercaptohexyl)pyridinium
Gold nanoparticles
Layer-by-layer deposition
Biomolecule adsorption
Bio-electro-catalysis
Electrochemical-quartz crystal
microbalance with dissipation
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/30190 Permalink to this page
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