Epitope approach in molecular imprinting of antibodies

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Epitope approach in molecular imprinting of antibodies

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Publication Article, peer reviewed scientific
Title Epitope approach in molecular imprinting of antibodies
Author Moczko, Ewa ; Guerreiro, Antonio ; Caceres, Cesar ; Piletska, Elena ; Sellergren, Börje ; Piletsky, Sergey A.
Date 2019
English abstract
Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMlPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope) onto the surface of a solid support, polymerization and affinity separation of nanoMlPs. The binding between nanoMIPs and their corresponding templates was analyzed and evaluated as being in sub-nanomolar and nano-molar range. The nanoMlPs prepared for Fc domain and epitope demonstrated a specific recognition of both human and goat IgGs, therefore they could be considered as a synthetic analogue of protein A and benefit from its intrinsic stability, short time and low cost of preparation.
DOI https://doi.org/10.1016/j.jchromb.2019.05.024 (link to publisher's fulltext.)
Publisher Elsevier
Host/Issue Journal of Chromatography. B;
Volume 1124
ISSN 1570-0232
Language eng (iso)
Subject Molecular imprinting polymers
Nanoparticles
Affinity chromatography
Solid-phase synthesis
Antibodies
Protein epitope
Sciences
Research Subject Categories::NATURAL SCIENCES
Handle http://hdl.handle.net/2043/30217 Permalink to this page
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