Epitope approach in molecular imprinting of antibodies

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Epitope approach in molecular imprinting of antibodies

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dc.contributor.author Moczko, Ewa
dc.contributor.author Guerreiro, Antonio
dc.contributor.author Caceres, Cesar
dc.contributor.author Piletska, Elena
dc.contributor.author Sellergren, Börje
dc.contributor.author Piletsky, Sergey A.
dc.date.accessioned 2019-10-14T14:11:52Z
dc.date.available 2019-10-14T14:11:52Z
dc.date.issued 2019 en_US
dc.identifier.issn 1570-0232 en_US
dc.identifier.uri http://hdl.handle.net/2043/30217
dc.description.abstract Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMlPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope) onto the surface of a solid support, polymerization and affinity separation of nanoMlPs. The binding between nanoMIPs and their corresponding templates was analyzed and evaluated as being in sub-nanomolar and nano-molar range. The nanoMlPs prepared for Fc domain and epitope demonstrated a specific recognition of both human and goat IgGs, therefore they could be considered as a synthetic analogue of protein A and benefit from its intrinsic stability, short time and low cost of preparation. en_US
dc.format.extent 6 en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.subject Molecular imprinting polymers en_US
dc.subject Nanoparticles en_US
dc.subject Affinity chromatography en_US
dc.subject Solid-phase synthesis en_US
dc.subject Antibodies en_US
dc.subject Protein epitope en_US
dc.subject.classification Sciences en_US
dc.title Epitope approach in molecular imprinting of antibodies en_US
dc.type Article, peer reviewed scientific en_US
dc.contributor.department Malmö University. Faculty of Health and Society
dc.contributor.department Malmö University. Biomedical Sciences (BMV)
dc.identifier.doi 10.1016/j.jchromb.2019.05.024 en_US
dc.subject.srsc Research Subject Categories::NATURAL SCIENCES en_US
dc.relation.ispartofpublication Journal of Chromatography. B;
dc.relation.ispartofpublicationvolume 1124 en_US
dc.description.authorversion No en_US
dc.identifier.isiid 000477685600001
dc.identifier.pmid 31176265
dc.format.ePage 6 en_US
dc.format.sPage 1 en_US
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