Electrochemical characterization and application of azurin-modified gold electrodes for detection of superoxide

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Electrochemical characterization and application of azurin-modified gold electrodes for detection of superoxide

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dc.contributor.author Ruzgas, Tautgirdas
dc.contributor.author Shleev, Sergey
dc.date.accessioned 2007-01-10T17:28:28Z
dc.date.available 2007-01-10T17:28:28Z
dc.date.issued 2006
dc.identifier.issn 0956-5663 en_US
dc.identifier.uri http://hdl.handle.net/2043/3289
dc.description.abstract A novel biosensor for superoxide radical (O2-) detection based on Pseudomonas aeruginosa azurin immobilized on gold electrode was designed. The rate constant of azurin reduction by O2- was found to be 105M−1 s−1 in solution and five times lower, i.e., 0.2x105M−1 s−1, for azurin coupled to gold by 3,3-dithiobis(sulfosuccinimidylpropionate) (DTSSP). The electron transfer rate between the protein and the electrode ranged from 2 to 6 s−1. The sensitivity of this biosensor to O2− was 6.8x102Am−2M−1. The response to the interference substances, such as uric acid, H2O2, and dimethylsulfoxide was negligible below 10 M. The electrode was applied in three O2− generating systems: (i) xanthine oxidase (XOD), (ii) potassium superoxide (KO2), and (iii) stimulated neutrophil granulocytes. The latter was compared with luminol-amplified chemiluminescence. The biosensor responded to O2− in all three environments, and the signals were antagonized by superoxide dismutase. en
dc.format.extent 7
dc.language.iso swe en
dc.subject Azurin en
dc.subject Superoxide en
dc.subject.classification Medicine en
dc.title Electrochemical characterization and application of azurin-modified gold electrodes for detection of superoxide en
dc.type Article, peer reviewed scientific en
dc.contributor.department Malmö University. Faculty of Health and Society en
dc.identifier.doi 10.1016/j.bios.2005.12.017
dc.subject.srsc Research Subject Categories::NATURAL SCIENCES en
dc.subject.srsc Research Subject Categories::NATURAL SCIENCES::Chemistry en
dc.relation.ispartofpublication Biosensors & Bioelectronics;2 en
dc.relation.ispartofpublicationvolume 22
dc.format.ePage 219
dc.format.sPage 213
mahlocal.rights.oaType green
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